The novel ovatoxin-g and isobaric palytoxin (so far referred to as putative palytoxin) from Ostreopsis cf. ovata (NW Mediterranean Sea): structural insights by LC-high resolution MSn

  1. García-Altares, María
  2. Tartaglione, Luciana
  3. Dell’Aversano, Carmela
  4. Carnicer, Olga 1
  5. de la Iglesia, Pablo
  6. Forino, Martino
  7. Diogène, Jorge
  8. Ciminiello, Patrizia
  1. 1 Universidad Politécnica de Cartagena
    info

    Universidad Politécnica de Cartagena

    Cartagena, España

    ROR https://ror.org/02k5kx966

Revista:
Analytical and Bioanalytical Chemistry

ISSN: 1618-2642,  1618-2650

Año de publicación: 2014

Volumen: 407

Número: 4

Páginas: 1191-1204

Tipo: Artículo

DOI: 10.1007/S00216-014-8338-Y GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Analytical and Bioanalytical Chemistry

Objetivos de desarrollo sostenible

Resumen

Blooms of the benthic dinoflagellate Ostreopsis cf. ovata are a concern in the Mediterranean Sea, since this species produces a wide range of palytoxin-like compounds listed among the most potent marine toxins. This study focused on two analogs of palytoxin found in cultures of six strains of O. cf. ovata isolated from the south of Catalonia (NW Mediterranean Sea). In addition to some already known ovatoxins, our strains produced two minor compounds, ovatoxin-g and the so far called putative palytoxin, whose structures had not been elucidated before. Insufficient quantity of these compounds impeded a full nuclear magnetic resonance (NMR)-based structural elucidation; thus, we studied their structure in crude algal extracts through liquid chromatography-electrospray ionization high-resolution mass spectrometry(n) (LC-ESI-HRMS(n)) in positive ion mode. Under the used MS conditions, the molecules underwent fragmentation at many sites of their backbone and a large number of diagnostic fragment ions were identified. As a result, tentative structures were assigned to both ovatoxin-g and the putative palytoxin, the latter being identified as a palytoxin isomer and re-named as isobaric palytoxin.

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