Elemental and macromolecular composition of the marine Chloropicophyceae, a major group of oceanic photosynthetic picoeukaryotes

  1. Ebenezer, Vinitha 2
  2. Hu, Yingyu 2
  3. Carnicer, Olga 1
  4. Irwin, Andrew J. 3
  5. Follows, Michael J.
  6. Finkel, Zoe V. 2
  1. 1 Department of Mathematics and Statistics Dalhousie University Halifax Nova Scotia Canada
  2. 2 Department of Oceanography Dalhousie University Halifax Nova Scotia Canada
  3. 3 Department of Earth Atmospheric and Planetary Science, Massachusetts Institute of Technology Cambridge Massachusetts USA
Mostrar afiliaciones +
Revista:
Limnology and Oceanography

ISSN: 0024-3590,  1939-5590

Año de publicación: 2022

Volumen: 67

Número: 3

Páginas: 540-551

Tipo: Artículo

DOI: 10.1002/LNO.12013 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Limnology and Oceanography

Objetivos de desarrollo sostenible

Resumen

Chloropicophyceae (Prasinophyte Clade VII) are small nonmotile coccoid cells with cell diameters ranging from 1 to 3 μm. Molecular surveys indicate they are relatively high in abundance in moderately oligotrophic oceanic waters and may substantively contribute to biogeochemical cycling in the sea. Here, we quantify the elemental and macromolecular composition of three subtropical Chloropicophyceae strains: Chloropicon mariensis, Chloropicon maureeniae, and Chloropicon roscoffensis under nutrient-sufficient exponential growth and nitrate starvation. Under nutrient-sufficient conditions the Chloropicophyceae are high in C : N and quite low in C : P and N : P relative to the canonical Redfield ratio, reflecting their relatively high nucleic acid composition compared to many other phytoplankton taxa. Nitrate starvation causes increases in C : N and C : P and decreases in N : P, primarily due to increases in carbohydrate and lipid and decreases in protein and RNA. There is genetic evidence that unlike most other green algae, Chloropicophyceae are diploid. The high nucleic acid content in the Chloropicon is consistent with the hypothesis that the nucleus, as a nonscalable component, takes up a larger and substantial proportion of cell mass in diploid picoeukaryotes. The elemental and macromolecular composition of these Chloropicophyceae, and relatively homeostatic response to N-starvation compared to diatoms, provides some insight into their success in the moderately oligotrophic ocean.

€ Ver financiación

Información de financiación

Financiadores

Referencias bibliográficas

  • 10.1007/s10895-008-0315-4
  • 10.1038/s41467-018-07346-z
  • 10.3989/scimar.2005.69n11
  • 10.1046/j.1529-8817.2001.01052.x
  • 10.4319/lo.2003.48.5.1721
  • 10.1038/s41467-017-02342-1
  • 10.1021/ac60119a033
  • 10.1046/j.1529-8817.1998.340844.x
  • Vries J., (2021), B.G., 18, pp. 1161
  • 10.1073/pnas.0604795103
  • 10.1002/cyto.a.10013
  • 10.1021/ac60111a017
  • Ebenezer V. Y. Y.Hu O.Carnicer A. J.Irwin M. J.Follows&Z. V.Finkel2021. Elemental and macromolecular composition of three species of Chloropicophyceae. Dataset deposited at Figshare.com. doi:10.6084/m9.figshare.14749524
  • 10.3354/ame010015
  • 10.1093/plankt/fbp098
  • 10.1371/journal.pone.0155977
  • 10.1016/S0021-9258(18)64849-5
  • 10.1364/AO.394123
  • 10.1128/AEM.64.9.3352-3358.1998
  • 10.1073/pnas.1423917112
  • 10.3389/fmicb.2018.00543
  • 10.1017/S0967026201003456
  • 10.1139/m62-029
  • 10.3390/metabo4020260
  • Holm‐Hansen O., (1973), Bull. Ecol. Commun., 17, pp. 215
  • Hu Y. Y.andZ. V.Finkel2020a. Inorganic polyphosphate in microalgae: A DAPI‐based quantification in microtiter plate. doi:10.17504/protocols.io.b3xkqpkw
  • Hu Y. Y.andZ. V.Finkel2020b. Lipids in microalgae: Quantitation by acid‐dichromate method in microtiter plate. doi:10.17504/protocols.io.bamiic4e
  • 10.3389/fmicb.2020.00086
  • 10.1093/plankt/fbi148
  • Jeffrey S. W., (1997), Phytoplankton pigments in oceanography: Guidelines to modern methods. Monographs on oceanographic methodology, pp. 449
  • 10.1038/ismej.2017.7
  • 10.1146/annurev-marine-010419-010706
  • 10.1111/j.1529-8817.1987.tb04217.x
  • 10.3390/genes11010066
  • 10.1038/s41467-019-12014-x
  • 10.1111/j.1462-2920.2009.02015.x
  • 10.1086/284372
  • 10.3389/fmicb.2019.00763
  • 10.1111/jpy.12376
  • 10.1038/ismej.2016.120
  • 10.1038/s41598-017-12412-5
  • 10.1016/B978-012088426-1/50018-4
  • 10.1128/AEM.02592-12
  • 10.1073/pnas.1321719111
  • 10.1038/ngeo1757
  • 10.1038/ismej.2010.104
  • 10.1186/gb-2012-13-8-r74
  • 10.1007/s11120-016-0310-6
  • 10.1080/09670260802578518
  • 10.1128/AEM.70.7.4064-4072.2004
  • 10.1016/j.cub.2008.09.039
  • 10.1073/pnas.0611046104
  • 10.1016/0003-2697(63)90094-0
  • 10.1098/rspb.2010.1356
  • Raven J. A., (1986), Can. J. Fish. Aquat. Sci., 214, pp. 1
  • 10.1093/plankt/16.5.565
  • 10.1046/j.1365-2435.1998.00233.x
  • 10.3389/fpls.2013.00536
  • Raven J. A., (2005), Vie Et Milieu, 55, pp. 209
  • Redfield A. C., (1934), James Johnstone memorial volume, pp. 177
  • 10.1038/nmeth.2089
  • 10.1128/AEM.02730-15
  • 10.4319/lo.1980.25.4.0754
  • Sterner R. W., (2002), Ecological stoichiometry: The biology of elements from molecules to the biosphere
  • 10.1111/j.1529-8817.1989.tb00266.x
  • 10.1016/S0304-4203(03)00054-9
  • 10.1038/s41598-019-41680-6
  • 10.1093/gbe/evz074
  • 10.1101/gr.253070.119
  • 10.4319/lo.2012.57.6.1877
  • 10.1002/9780470281840.ch6
  • 10.1126/science.1167222
  • 10.1073/pnas.1906897116