Construction and comparative analyses of highly dense linkage maps of two sweet cherry intraspecific progenies of commercial cultivars

  1. C. Klagges
  2. Campoy J.A.
  3. Quero‐García J.
  4. Guzmán A.
  5. Mansur L.
  6. Gratacós E.
  7. Silva H.
  8. Rosyara U.R.
  9. Iezzoni A.
  10. Meisel L.A.
  11. Dirlewanger E.
Aktak:
6th International Rosaceae Genomics Conference (RGC6)

Argitalpen urtea: 2012

Mota: Biltzarraren Kartela

GOOGLE SCHOLAR

Laburpena

Sweet cherry (Prunus avium) is an important fruit tree crop. The construction of a sweet cherry genetic linkage map is important to facilitate quantitative trait locus analyses and gene tagging for marker assisted breeding programs. In this work, high‐density genetic maps for four parents and two F1 populations obtained from two intra‐specific crosses between ‘Black Tartarian’ × ‘Kordia’ (BT×K) and ‘Regina’ × ‘Lapins’(R×L) were constructed using the recently designed RosBREED 6K SNP chip. A total of 5,696 SNP markers were tested in each progeny, mapping 723 SNPs and 701 SNPs in the BT×K and R×L linkage maps, respectively. The resulting maps spanned 752.9 and 651.6 cM with an average distance of 1.1 and 0.9 cM between adjacent markers. The maps displayed high synteny and co‐linearity between each other and with the peach genome v1.0 for all eight linkage groups (LG1‐LG8). However, we identified inversions in sweet cherry genome when compared to the peach genome at LG1, LG5 and LG7. The highly dense linkage maps developed from these two segregating populations of contrasting cherry cultivars will be useful for further research in development of markers for economically important traits, based upon the haplotype segregation in the progeny.

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Funded by 07CN13PBT‐167 INNOVA Chile CORFO project, INRA’s Department of ‘Genetic and Plant Breeding’ and the Conseil Régional d'Aquitaine 2011 project ‘convention 2011‐12 01 00122000568’, UNAB DI‐78‐12/I project, the USDA’s National Institute of Food and Agriculture – Specialty Crop Research Initiative project “RosBREED: Enabling marker‐assisted breeding in Rosaceae” (2009‐51181‐05808), Fondecyt Nº1120261 and N°1121021. CONICYT doctoral fellowship (N°21120115 and N°24121484). Postdoctoral fellowship from the “Programa de Formación Postdoctoral de Personal Investigador” of the “Fundación Séneca‐Agencia de Ciencia y Tecnología de la Región de Murcia”, Spain.