A Review of Virtual Inertia Techniques for Renewable Energy-Based Generators

  1. Fernández-Guillamón, Ana
  2. Gómez-Lázaro, Emilio
  3. Muljadi, Eduard
  4. Molina-Garcia, Ángel
Libro:
Renewable Energy - Technologies and Applications

Editorial: IntechOpen

ISBN: 978-1-83881-001-6

Año de publicación: 2021

Tipo: Capítulo de Libro

DOI: 10.5772/INTECHOPEN.92651 GOOGLE SCHOLAR lock_openAcceso abierto editor

Objetivos de desarrollo sostenible

Resumen

Over recent decades, the penetration of renewable energy sources (RES), especially photovoltaic and wind power plants, has been promoted in most countries. However, as these both alternative sources have power electronics at the grid interface (inverters), they are electrically decoupled from the grid. Subsequently, stability and reliability of power systems are compromised. Inertia in power systems has been traditionally determined by considering all the rotating masses directly connected to the grid. Thus, as the penetration of renewable units increases, the inertia of the power system decreases due to the reduction of directly connected rotating machines. As a consequence, power systems require a new set of strategies to include these renewable sources. In fact, ‘hidden inertia,’ ‘synthetic inertia’ and ‘virtual inertia’ are terms currently used to represent an artificial inertia created by inverter control strategies of such renewable sources. This chapter reviews the inertia concept and proposes a method to estimate the rotational inertia in different parts of the world. In addition, an extensive discussion on wind and photovoltaic power plants and their contribution to inertia and power system stability is presented.

Referencias bibliográficas

  • Babahajiani P, Shafiee Q, Bevrani H. Intelligent demand response contribution in frequency control of multi-area power systems. IEEE Transactions on Smart Grid. 2018;9(2):1282-1291
  • D’hulst R, Fernandez JM, Rikos E, Kolodziej D, Heussen K, Geibelk D, et al. Voltage and frequency control for future power systems: The ELECTRA IRP proposal. In: 2015 International Symposium on Smart Electric Distribution Systems and Technologies (EDST), IEEE. 2015. pp. 245-250
  • Fernández-Guillamón A, Das K, Cutululis NA, Molina-García Á. Offshore wind power integration into future power systems: Overview and trends. Journal of Marine Science and Engineering. 2019;7(11):399
  • Shah R, Mithulananthan N, Bansal R, Ramachandaramurthy V. A review of key power system stability challenges for large-scale PV integration. Renewable and Sustainable Energy Reviews. 2015;41(Supplement C):1423-1436
  • Cvetković M, Pan K, López CD, Bhandia R, Palensky P. Co-simulation aspects for energy systems with high penetration of distributed energy resources. In: AEIT International Annual Conference; 2016. IEEE. 2017. pp. 1-6
  • Wang Y, Meng J, Zhang X, Xu L. Control of pmsg-based wind turbines for system inertial response and power oscillation damping. IEEE Transactions on Sustainable Energy. 2015;6(2):565-574
  • Junyent-Ferr A, Pipelzadeh Y, Green TC. Blending hvdc-link energy storage and offshore wind turbine inertia for fast frequency response. IEEE Transactions on Sustainable Energy. 2015;6(3):1059-1066
  • Yang S, Fang J, Tang Y, Qiu H, Dong C, Wang P. Synthetic-inertia-based modular multilevel converter frequency control for improved micro-grid frequency regulation. In: 2018 IEEE Energy Conversion Congress and Exposition (ECCE); IEEE. 2018. pp. 5177-5184
  • Delille G, Francois B, Malarange G. Dynamic frequency control support by energy storage to reduce the impact of wind and solar generation on isolated power system’s inertia. IEEE Transactions on Sustainable Energy. 2012;3(4):931-939
  • Fernández-Guillamón A, Gómez-Lázaro E, Muljadi E, Molina-García Á. Power systems with high renewable energy sources: A review of inertia and frequency control strategies over time. Renewable and Sustainable Energy Reviews. 2019;115:109369
  • Dehghanpour K, Afsharnia S. Electrical demand side contribution to frequency control in power systems: A review on technical aspects. Renewable and Sustainable Energy Reviews. 2015;41:1267-1276
  • Nguyen HT, Yang G, Nielsen AH, Jensen PH. Combination of synchronous condenser and synthetic inertia for frequency stability enhancement in low inertia systems. IEEE Transactions on Sustainable Energy. 2018;10(3):997-1005
  • Groß D, Bolognani S, Poolla BK, Dörfler F. Increasing the resilience of low-inertia power systems by virtual inertia and damping. In: Bulk Power Systems Dynamics and Control Symposium (IREP). 2017
  • Ustun TS, Aoto Y. Analysis of smart inverter’s impact on the distribution network operation. IEEE Access. 2019;7:9790-9804
  • Vokony I. Effect of inertia deficit on power system stability-synthetic inertia concepts analysis. In: 2017 6th International Youth Conference on Energy (IYCE); IEEE. 2017. pp. 1-6
  • Ulbig A, Borsche TS, Andersson G. Impact of low rotational inertia on power system stability and operation. IFAC Proceedings Volumes. 2014;47(3):7290-7297
  • Serway RA, Jewett JW. Physics for Scientists and Engineers with Modern Physics. Cengage Learning: Brooks/Cole; 2018
  • Uriarte FM, Smith C, Van Broekhoven S, Hebner RE. Microgrid ramp rates and the inertial stability margin. IEEE Transactions on Power Systems. 2015;30(6):3209-3216
  • Fernández-Guillamón A, Vigueras-Rodríguez A, Molina-García A. Análisis y simulación de estrategias agregadas de control de frecuencia entre grandes parques eólicos y aprovechamientos hidroélectricos [MS thesis]. Universidad Politécnica de Cartagena; 2017
  • Huang H, Li F. Sensitivity analysis of load-damping characteristic in power system frequency regulation. IEEE Transactions on Power Systems. 2013;28(2):1324-1335
  • Tielens P, Van Hertem D. The relevance of inertia in power systems. Renewable and Sustainable Energy Reviews. 2016;55:999-1009
  • Fernández-Guillamón A, Vigueras-Rodríguez A, Molina-García Á. Analysis of power system inertia estimation in high wind power plant integration scenarios. IET Renewable Power Generation. 2019;13(15):2807-2816
  • Muñoz-Benavente I, Hansen AD, Gómez-Lazaro E, García-Sánchez T, Fernández-Guillamón A, Molina-García Á. Impact of combined demand-response and wind power plant participation in frequency control for multi-area power systems. Energies. 2019;12(9):1687
  • Gu H, Yan R, Saha TK. Minimum synchronous inertia requirement of renewable power systems. IEEE Transactions on Power Systems. 2017;33(2):1533-1543
  • Tielens P, Van Hertem D. Receding horizon control of wind power to provide frequency regulation. IEEE Transactions on Power Systems. 2017;32(4):2663-2672
  • Kroposki B, Johnson B, Zhang Y, Gevorgian V, Denholm P, Hodge B-M, et al. Achieving a 100% renewable grid: Operating electric power systems with extremely high levels of variable renewable energy. IEEE Power and Energy Magazine. 2017;15(2):61-73
  • Du P, Matevosyan J. Forecast system inertia condition and its impact to integrate more renewables. IEEE Transactions on Smart Grid. 2018;9(2):1531-1533
  • Muyeen S, Takahashi R, Murata T, Tamura J. A variable speed wind turbine control strategy to meet wind farm grid code requirements. IEEE Transactions on Power Systems. 2010;25(1):331-340
  • Zhao J, Lyu X, Fu Y, Hu X, Li F. Coordinated microgrid frequency regulation based on DFIG variable coefficient using virtual inertia and primary frequency control. IEEE Transactions on Energy Conversion. 2016;31(3):833-845
  • Hosseinipour A, Hojabri H. Virtual inertia control of PV systems for dynamic performance and damping enhancement of dc microgrids with constant power loads. IET Renewable Power Generation. 2017;12(4):430-438
  • Tielens P. Operation and control of power systems with low synchronous inertia [PhD thesis]. KU Leuven; 2017
  • Yingcheng X, Nengling T. Review of contribution to frequency control through variable speed wind turbine. Renewable Energy. 2011;36(6):1671-1677
  • Fischer M, Engelken S, Mihov N, Mendonca A. Operational experiences with inertial response provided by type 4 wind turbines. IET Renewable Power Generation. 2016;10(1):17-24
  • Tang ZX, Lim YS, Morris S, Yi JL, Lyons PF, Taylor PC. A comprehensive work package for energy storage systems as a means of frequency regulation with increased penetration of photovoltaic systems. International Journal of Electrical Power & Energy Systems. 2019;110:197-207
  • Yang L, Hu Z, Xie S, Kong S, Lin W. Adjustable virtual inertia control of supercapacitors in PV-based ac microgrid cluster. Electric Power Systems Research. 2019;173:71-85
  • Li W, Du P, Lu N. Design of a new primary frequency control market for hosting frequency response reserve offers from both generators and loads. IEEE Transactions on Smart Grid. 2017;9(5):4883-4892
  • You R, Barahona B, Chai J, Cutululis NA, Wu X. Improvement of grid frequency dynamic characteristic with novel wind turbine based on electromagnetic coupler. Renewable Energy. 2017;113:813-821
  • Attya A, Dominguez-Garcia J, Anaya-Lara O. A review on frequency support provision by wind power plants: Current and future challenges. Renewable and Sustainable Energy Reviews. 2018;81:2071-2087
  • Wang S, Tomsovic K. A novel active power control framework for wind turbine generators to improve frequency response. IEEE Transactions on Power Systems. 2018;33(6):6579-6589
  • Ziping W, Wenzhong G, Tianqi G, Weihang Y, Zhang H, Shijie Y, et al. State-of-the-art review on frequency response of wind power plants in power systems. Journal of Modern Power Systems and Clean Energy. 2018;6(1):1-16
  • International Energy Agency. Total primary energy supply (TPES) by source, year and country. Available from: https://bit.ly/34YTcda. [Accessed: 17 October 2018]
  • ENTSO-E. High Penetration of Power Electronic Interfaced Power Sources (HPoPEIPS). Available from: https://bit.ly/2x5fZrh
  • Fernández-Guillamón A, Sarasúa JI, Chazarra M, Vigueras-Rodríguez A, Fernández-Muñoz D, Molina-García Á. Frequency control analysis based on unit commitment schemes with high wind power integration: A Spanish isolated power system case study. International Journal of Electrical Power Energy Systems. 2020;121:106044
  • Bevrani H, Daneshmand PR. Fuzzy logic-based load-frequency control concerning high penetration of wind turbines. IEEE Systems Journal. 2012;6(1):173-180
  • Ozer B, Arikan O, Moral G, Altintas A. Extraction of primary and secondary frequency control from active power generation data of power plants. International Journal of Electrical Power & Energy Systems. 2015;73:16-22
  • Nedd M, Booth C, Bell K. Potential solutions to the challenges of low inertia power systems with a case study concerning synchronous condensers. In: 2017 52nd International Universities Power Engineering Conference (UPEC); IEEE. 2017. pp. 1-6
  • Wang X, Yue M. Design of energy storage system to improve inertial response for large scale PV generation. In: 2016 IEEE Power and Energy Society General Meeting (PESGM). 2016. pp. 1-5
  • Luo X, Wang J, Dooner M, Clarke J. Overview of current development in electrical energy storage technologies and the application potential in power system operation. Applied Energy. 2015;137:511-536
  • Chen H, Cong TN, Yang W, Tan C, Li Y, Ding Y. Progress in electrical energy storage system: A critical review. Progress in Natural Science. 2009;19(3):291-312
  • Akram U, Nadarajah M, Shah R, Milano F. A review on rapid responsive energy storage technologies for frequency regulation in modern power systems. Renewable and Sustainable Energy Reviews. 2020;120:109626
  • Marcos J, Storkël O, Marroyo L, Garcia M, Lorenzo E. Storage requirements for PV power ramp-rate control. Solar Energy. 2014;99:28-35
  • Salim NB, Aboelsoud H, Tsuji T, Oyama T, Uchida K. Load frequency control of two-area network using renewable energy resources and battery energy storage system. Journal of Electrical Systems. 2017;13(2):348-365
  • Zhao Z, Xiao H, Yang Y. Improved coordinated control strategy of hybrid energy storages in PV power smoothing. Energy Procedia. 2018;145:151-156
  • Cabrane Z, Ouassaid M, Maaroufi M. Analysis and evaluation of battery-supercapacitor hybrid energy storage system for photovoltaic installation. International Journal of Hydrogen Energy. 2016;41(45):20897-20907
  • Chandra A. Supercapacitors: An alternate technology for energy storage. Proceedings of the National Academy of Sciences. 2012;82:79-90
  • Taghizadeh M, Hoseintabar M, Faiz J. Frequency control of isolated WT/PV/SOFC/UC network with new control strategy for improving SOFC dynamic response. International Transactions on Electrical Energy Systems. 2015;25(9):1748-1770
  • You S, Liu Y, Tan J, Gonzalez MT, Zhang X, Zhang Y, et al. Comparative assessment of tactics to improve primary frequency response without curtailing solar output in high photovoltaic interconnection grids. IEEE Transactions on Sustainable Energy. 2018;10(2):718-728
  • Mousavi GS, Faraji F, Majazi A, Al-Haddad K. A comprehensive review of flywheel energy storage system technology. Renewable and Sustainable Energy Reviews. 2017;67:477-490
  • Amiryar ME, Pullen KR. A review of flywheel energy storage system technologies and their applications. Applied Sciences. 2017;7:286(1-21)
  • Pullen KR. The status and future of flywheel energy storage. Joule. 2019;3(6):1394-1399
  • Akinyele D, Rayudu R. Review of energy storage technologies for sustainable power networks. Sustainable Energy Technologies and Assessments. 2014;8:74-91
  • Barelli L, Bidini G, Bonucci F, Castellini L, Fratini A, Gallorini F, et al. Flywheel hybridization to improve battery life in energy storage systems coupled to res plants. Energy. 2019;173:937-950
  • Xin H, Liu Y, Wang Z, Gan D, Yang T. A new frequency regulation strategy for photovoltaic systems without energy storage. IEEE Transactions on Sustainable Energy. 2013;4(4):985-993
  • Alatrash H, Mensah A, Mark E, Haddad G, Enslin J. Generator emulation controls for photovoltaic inverters. IEEE Transactions on Smart Grid. 2012;3(2):996-1011
  • Zarina P, Mishra S, Sekhar P. Deriving inertial response from a non-inertial PV system for frequency regulation. In: 2012 IEEE International Conference on Power Electronics, Drives and Energy Systems (PEDES); IEEE. 2012. pp. 1-5
  • Zarina P, Mishra S, Sekhar P. Photovoltaic system based transient mitigation and frequency regulation. In: 2012 Annual IEEE India Conference (INDICON); IEEE. 2012. pp. 1245-1249
  • García-Gracia M, El Halabi N, Ajami H, Comech MP. Integrated control technique for compliance of solar photovoltaic installation grid codes. IEEE Transactions on Energy Conversion. 2012;27(3):792-798
  • Moutis P, Vassilakis A, Sampani A, Hatziargyriou N. DC switch driven active power output control of photovoltaic inverters for the provision of frequency regulation. IEEE Transactions on Sustainable Energy. 2015;6(4):1485-1493
  • Mishra S, Zarina P, Sekhar P. A novel controller for frequency regulation in a hybrid system with high PV penetration. In: 2013 IEEE Power and Energy Society General Meeting (PES); IEEE. 2013. pp. 1-5
  • Zarina P, Mishra S, Sekhar P. Exploring frequency control capability of a PV system in a hybrid PV-rotating machine-without storage system. International Journal of Electrical Power & Energy Systems. 2014;60:258-267
  • Ziping W, Wenzhong G, Tianqi G, Weihang Y, ZHANG H, Shijie Y, et al. State-of-the-art review on frequency response of wind power plants in power systems. Journal of Modern Power Systems and Clean Energy. 2017:1-16
  • Xiong L, Li Y, Zhu Y, Yang P, Xu Z. Coordinated control schemes of super-capacitor and kinetic energy of DFIG for system frequency support. Energies. 2018;11(1):103
  • Jauch C, Hippel S. Hydraulic–pneumatic flywheel system in a wind turbine rotor for inertia control. IET Renewable Power Generation. 2016;10(1):33-41
  • Wen J, Liu J, Long Y, Yao W. Solution to short-term frequency response of wind farms by using energy storage systems. IET Renewable Power Generation. May 2016;10:669-678
  • Gonzalez-Longatt FM, Alhejaj SM. Enabling inertial response in utility-scale battery energy storage system. In: 2016 IEEE Innovative Smart Grid Technologies-Asia (ISGT-Asia). 2016. pp. 605-610
  • Tan J, Zhang Y. Coordinated control strategy of a battery energy storage system to support a wind power plant providing multi-timescale frequency ancillary services. IEEE Transactions on Sustainable Energy. 2017;8(3):1140-1153
  • He G, Chen Q, Kang C, Xia Q, Poolla K. Cooperation of wind power and battery storage to provide frequency regulation in power markets. IEEE Transactions on Power Systems. 2017;32(5):3559-3568
  • Bai L, Li F, Hu Q, Cui H, Fang X. Application of battery-supercapacitor energy storage system for smoothing wind power output: An optimal coordinated control strategy. In: 2016 IEEE Power and Energy Society General Meeting (PESGM). 2016. pp. 1-5
  • Tan Y, Muttaqi KM, Ciufo P, Meegahapola L. Enhanced frequency response strategy for a pmsg-based wind energy conversion system using ultracapacitor in remote area power supply systems. IEEE Transactions on Industry Applications. 2017;53(1):549-558
  • Gayathri NS, Kar IN. Smoothing of wind power using flywheel energy storage system. IET Renewable Power Generation. 2017;11:289-298
  • Díaz-González F, Sumper A, Gomis-Bellmunt O, Bianchi FD. Energy management of flywheel-based energy storage device for wind power smoothing. Applied Energy. 2013;110:207-219
  • Yao J, Yu M, Gao W, Zeng X. Frequency regulation control strategy for PMSG wind-power generation system with flywheel energy storage unit. IET Renewable Power Generation. June 2017;11:1082-1093
  • Zhao H, Wu Q, Hu S, Xu H, Rasmussen CN. Review of energy storage system for wind power integration support. Applied Energy. 2015;137:545-553
  • Díaz-González F, Hau M, Sumper A, Gomis-Bellmunt O. Coordinated operation of wind turbines and flywheel storage for primary frequency control support. International Journal of Electrical Power Energy Systems. 2015;68:313-326
  • Ahmadi R, Ghardashi F, Kabiri D, Sheykholeslami A, Haeri H. Voltage and frequency control in smart distribution systems in presence of der using flywheel energy storage system. IET Conference Proceedings. January 2013:1307-1307
  • Ghosh S, Kamalasadan S. An energy function-based optimal control strategy for output stabilization of integrated DFIG-flywheel energy storage system. IEEE Transactions on Smart Grid. 2017;8(4):1922-1931
  • Zhang X, Zha X, Yue S, Chen Y. A frequency regulation strategy for wind power based on limited over-speed de-loading curve partitioning. IEEE Access. 2018;6:22938-22951
  • Moutis P, Loukarakis E, Papathanasiou S, Hatziargyriou ND. Primary load-frequency control from pitch-controlled wind turbines. In: 2009 IEEE Bucharest PowerTech; IEEE. 2009. pp. 1-7
  • Ma H, Chowdhury B. Working towards frequency regulation with wind plants: Combined control approaches. IET Renewable Power Generation. 2010;4(4):308-316
  • Moutis P, Papathanassiou SA, Hatziargyriou ND. Improved load-frequency control contribution of variable speed variable pitch wind generators. Renewable Energy. 2012;48:514-523
  • Žertek A, Verbič G, Pantoš M. Optimised control approach for frequency-control contribution of variable speed wind turbines. IET Renewable Power Generation. 2012;6(1):17-23
  • Castro LM, Fuerte-Esquivel CR, Tovar-Hernández JH. Solution of power flow with automatic load-frequency control devices including wind farms. IEEE Transactions on Power Systems. 2012;27(4):2186-2195
  • Vidyanandan K, Senroy N. Primary frequency regulation by deloaded wind turbines using variable droop. IEEE Transactions on Power Systems. 2013;28(2):837-846
  • Alsharafi AS, Besheer AH, Emara HM. Primary frequency response enhancement for future low inertia power systems using hybrid control technique. Energies. 2018;11(4):699
  • Ye H, Pei W, Qi Z. Analytical modeling of inertial and droop responses from a wind farm for short-term frequency regulation in power systems. IEEE Transactions on Power Systems. 2016;31(5):3414-3423
  • Fakhari Moghaddam Arani M, Mohamed YAI. Dynamic droop control for wind turbines participating in primary frequency regulation in microgrids. IEEE Transactions on Smart Grid. 2018;9(6):5742-5751
  • Lertapanon P, Wangdee W. Analysis and modeling of wind turbine generators considering frequency controls. In: 2017 International Electrical Engineering Congress (iEECON); IEEE. 2017. pp. 1-4
  • Huang L, Xin H, Zhang L, Wang Z, Wu K, Wang H. Synchronization and frequency regulation of DFIG-based wind turbine generators with synchronized control. IEEE Transactions on Energy Conversion. 2017;32(3):1251-1262
  • Deepak M, Abraham RJ, Gonzalez-Longatt FM, Greenwood DM, Rajamani H-S. A novel approach to frequency support in a wind integrated power system. Renewable Energy. 2017;108:194-206
  • Gonzalez-Longatt F, Chikuni E, Stemmet W, Folly K. Effects of the synthetic inertia from wind power on the total system inertia after a frequency disturbance. In: Power Engineering Society Conference and Exposition in Africa; Citeseer. 2012. pp. 9-13
  • Bonfiglio A, Invernizzi M, Labella A, Procopio R. Design and implementation of a variable synthetic inertia controller for wind turbine generators. IEEE Transactions on Power Systems. 2019;34(1):754-764
  • Liu K, Qu Y, Kim H-M, Song H. Avoiding frequency second dip in power unreserved control during wind power rotational speed recovery. IEEE Transactions on Power Systems. 2018;33(3):3097-3106
  • Morren J, de Haan SWH, Kling WL, Ferreira JA. Wind turbines emulating inertia and supporting primary frequency control. IEEE Transactions on Power Systems. February 2006;21:433-434
  • Díaz-González F, Hau M, Sumper A, Gomis-Bellmunt O. Participation of wind power plants in system frequency control: Review of grid code requirements and control methods. Renewable and Sustainable Energy Reviews. 2014;34:551-564
  • Dreidy M, Mokhlis H, Mekhilef S. Inertia response and frequency control techniques for renewable energy sources: A review. Renewable and Sustainable Energy Reviews. 2017;69:144-155
  • Tarnowski GC, Kjar PC, Sorensen PE, Ostergaard J. Variable speed wind turbines capability for temporary over-production. In: Power & Energy Society General Meeting, 2009. PES’09. IEEE. 2009. pp. 1-7
  • Keung P-K, Li P, Banakar H, Ooi BT. Kinetic energy of wind-turbine generators for system frequency support. IEEE Transactions on Power Systems. 2009;24(1):279-287
  • El Itani S, Annakkage UD, Joos G. Short-term frequency support utilizing inertial response of DFIG wind turbines. In: 2011 IEEE Power and Energy Society General Meeting; IEEE. 2011. pp. 1-8
  • Hansen AD, Altin M, Margaris ID, Iov F, Tarnowski GC. Analysis of the short-term overproduction capability of variable speed wind turbines. Renewable Energy. 2014;68:326-336
  • Hafiz F, Abdennour A. Optimal use of kinetic energy for the inertial support from variable speed wind turbines. Renewable Energy. 2015;80:629-643
  • Kang M, Kim K, Muljadi E, Park J-W, Kang YC. Frequency control support of a doubly-fed induction generator based on the torque limit. IEEE Transactions on Power Systems. 2016;31(6):4575-4583
  • Fernández-Guillamón A, Villena-Lapaz J, Vigueras-Rodríguez A, García-Sánchez T, Molina-García Á. An adaptive frequency strategy for variable speed wind turbines: Application to high wind integration into power systems. Energies. 2018;11(6):1-21
  • Liu K, Qu Y, Kim H-M, Song H. Avoiding frequency second dip in power unreserved control during wind power rotational speed recovery. IEEE Transactions on Power Systems. 2017;33(3):3097-3106
  • Fernández-Guillamón A, Vigueras-Rodríguez A, Gómez-Lázaro E, Molina-García Á. Fast power reserve emulation strategy for VSWT supporting frequency control in multi-area power systems. Energies. 2018;11(10):2775(1-20)
  • Wu Z, Gao DW, Zhang H, Yan S, Wang X. Coordinated control strategy of battery energy storage system and PMSG-WTG to enhance system frequency regulation capability. IEEE Transactions on Sustainable Energy. 2017;8(3):1330-1343