Using 23S rDNA to identify contaminations of Escherichia coli in Agrobacterium tumefaciens cultures

  1. Manchado Rojo, María
  2. Weiss, Julia Rosl
  3. Egea Gutiérrez-Cortines, Marcos
Libro:
Proceedings of the 4th. Workshop on Agri-Food Research. WIA.15
  1. Artés Hernández, Francisco
  2. Egea Gutíerrez-Cortines, Marcos
  3. Fernández Hernández, Juan Antonio
  4. Baille, Alain
  5. Calatrava Leyva, Javier (coord.)

Editorial: Universidad Politécnica de Cartagena

ISBN: 978-84-608-5399-2

Año de publicación: 2016

Páginas: 90-92

Congreso: Workshop on Agri-Food Research for young researchers (4. 2015. Cartagena)

Tipo: Aportación congreso

DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Resumen

Cross contamination of Agrobacterium tumefaciens stocks with Escherichia coli are difficult to identify by microbiological techniques, leading to false negative results in transformation experiments. We have developed a genotyping assay for A. tumefaciens and E. coli lab strains based on amplification of 23S rDNA by PCR. Agrobacterium strains LBA 4404, C58 and EHA105 and E. coli DB3.1, DH5α and XL1-Blue can be identified separating the corresponding PCR amplicons in 2.5% agarose gels. However in crossed contaminations, interpretation of results is improved using melting point analysis on a quantitative PCR machine.

Fuente de los datos: Dialnet