Análisis de la función génica mediante delecciones por CRISPR / Cas9 y transcriptómicas

  1. Nmichi, N
  2. Badaoui, B.
  3. Weiss, Julia Rosl
  4. Egea Gutiérrez-Cortines, Marcos
Book:
Proceedings of the 8th Workshop on agri-food research. WIA.19

Publisher: Universidad Politécnica de Cartagena

ISBN: 978-84-17853-08-2

Year of publication: 2020

Pages: 164-167

Congress: Workshop on Agri-Food Research for young researchers (8. 2019. Cartagena)

Type: Conference paper

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Abstract

CRISPR-Cas9, a revolutionary technique currently used to modify the genome in a precise way, is based on DNA repair mechanisms activated locally by DNA breakage. CRISPR is associated with an endonuclease called Cas9. This complex is directed by a gRNA (guide) conferring specificity of action to a given DNA sequence. The aim of the present work is to use the CRISPR / Cas9 technique to develop a series of mutagenetic plants using in vitro transformation.

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