Análisis de la función génica mediante delecciones por CRISPR / Cas9 y transcriptómicas
- Nmichi, N
- Badaoui, B.
- Weiss, Julia Rosl
- Egea Gutiérrez-Cortines, Marcos
Éditorial: Universidad Politécnica de Cartagena
ISBN: 978-84-17853-08-2
Année de publication: 2020
Pages: 164-167
Congreso: Workshop on Agri-Food Research for young researchers (8. 2019. Cartagena)
Type: Communication dans un congrès
Résumé
CRISPR-Cas9, a revolutionary technique currently used to modify the genome in a precise way, is based on DNA repair mechanisms activated locally by DNA breakage. CRISPR is associated with an endonuclease called Cas9. This complex is directed by a gRNA (guide) conferring specificity of action to a given DNA sequence. The aim of the present work is to use the CRISPR / Cas9 technique to develop a series of mutagenetic plants using in vitro transformation.