Diseño de ARN guía para la edición del gen Gigantea en Solanáceas
- Brandoli, Claudio
- Petri Serrano, César
- Weiss, Julia Rosl
- Francisco Artés Hernández
- Juan Antonio Fernández-Hernández
- José Enrique Cos Terrer
- Juan José Alarcón Cabañero
- Marcos Egea Gutiérrez-Cortines
- Encarna Aguayo Giménez
Éditorial: Universidad Politécnica de Cartagena
ISBN: 978-84-16325-89-4
Année de publication: 2019
Pages: 121-124
Congreso: Workshop on Agri-Food Research for young researchers (7. 2018. Cartagena)
Type: Communication dans un congrès
Résumé
The CRISPR/Cas9 system nowadays has become the most used genome editing technology in the scientific world. Although this technique is in constant evolution, one of the main problems that persists is the lack of solid bioinformatics tools for the design of guide-RNAs (gRNA) for different species with high effectiveness and specificity. Here, we describe the methodology of design and generation of gRNA for genome editing through CRISPR/Cas9 technology in Solanaceae.